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101.
Brockhurst MA Buckling A Poullain V Hochberg ME 《Evolution; international journal of organic evolution》2007,61(5):1238-1243
Natural populations of hosts and parasites are often subdivided and patchily distributed such that some regions of a host species' range will be free from a given parasite. Host migration from parasite-free to parasite-containing patches is expected to alter coevolutionary dynamics by changing the evolutionary potential of antagonists. Specifically, host immigration can favor parasites by increasing transmission opportunities, or hosts by introducing genetic variation. We tested these predictions in coevolving populations of Pseudomonas fluorescens and phage Phi2 that received immigrants from phage-free populations. We observed a negative quadratic relationship between sympatric resistance to phage and host immigration rate (highest at intermediate immigration) but a positive quadratic relationship between coevolution rate and host immigration rate (lowest at intermediate immigration). These results indicate that for a wide range of rates, host immigration from parasite-free patches can increase the evolutionary potential of parasites, and increase the coevolutionary rate if parasite adaptation is limiting in the absence of immigration. 相似文献
102.
Imad J. Matouk Eli Raveh Rasha Abu-lail Shaul Mezan Michal Gilon Eitan Gershtain Tatiana Birman Jennifer Gallula Tamar Schneider Moshe Barkali Carmelit Richler Yakov Fellig Vladimir Sorin Ayala Hubert Abraham Hochberg Abraham Czerniak 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》2014
The oncofetal H19 gene transcribes a long non-coding RNA(lncRNA) that is essential for tumor growth. Here we found that numerous established inducers of epithelial to mesenchymal transition(EMT) also induced H19/miR-675 expression. Both TGF-β and hypoxia concomitantly induced H19 and miR-675 with the induction of EMT markers. We identified the PI3K/AKT pathway mediating the inductions of Slug, H19 RNA and miR-675 in response to TGF-β treatment, while Slug induction depended on H19 RNA. In the EMT induced multidrug resistance model, H19 level was also induced. In a mouse breast cancer model, H19 expression was tightly correlated with metastatic potential. In patients, we detected high H19 expression in all common metastatic sites tested, regardless of tumor primary origin. H19 RNA suppressed the expression of E-cadherin protein. H19 up-regulated Slug expression concomitant with the suppression of E-cadherin protein through a mechanism that involved miR-675. Slug also up-regulated H19 expression and activated its promoter. Altogether, these results may support the existence of a positive feedback loop between Slug and H19/miR-675, that regulates E-cadherin expression. H19 RNA enhanced the invasive potential of cancer cells in vitro and enhanced tumor metastasis in vivo. Additionally, H19 knockdown attenuated the scattering and tumorigenic effects of HGF/SF. Our results present novel mechanistic insights into a critical role for H19 RNA in tumor progression and indicate a previously unknown link between H19/miR-675, Slug and E-cadherin in the regulation of cancer cell EMT programs. 相似文献
103.
Timothée Poisot James D. Bever Peter H. Thrall Michael E. Hochberg 《Ecology and evolution》2014,4(19):3841-3850
Protective mutualisms, where a symbiont reduces the negative effects of another species on a shared host, represent a common type of species interaction in natural communities, yet it is still unclear what ecological conditions might favor their emergence. Studies suggest that the initial evolution of protective mutualists might involve closely related pathogenic variants with similar life histories, but different competitive abilities and impacts on host fitness. We derive a model to evaluate this hypothesis and show that, in general, a protective variant cannot spread from rarity or exclude a more pathogenic strain. While the conditions allowing mutualist invasion are more likely with increased environmental productivity, they still depend on initial densities in the invaded patch exceeding a threshold, highlighting the likely importance of spatial structure and demographic stochasticity. Using a numerical simulation approach, we show that regional coexistence is in fact possible in an explicitly spatial system and that, under some circumstances, the mutualist population can exclude the enemy. More broadly, the establishment of protective mutualists may be favored when there are other life‐history differences from more pathogenic symbionts, such as vertical transmission or additional direct benefits to hosts. 相似文献
104.
The evolution of specificity in evolving and coevolving antagonistic interactions between a bacteria and its phage 总被引:1,自引:0,他引:1
Poullain V Gandon S Brockhurst MA Buckling A Hochberg ME 《Evolution; international journal of organic evolution》2008,62(1):1-11
The evolution of exploitative specificity can be influenced by environmental variability in space and time and the intensity of trade-offs. Coevolution, the process of reciprocal adaptation in two or more species, can produce variability in host exploitation and as such potentially drive patterns in host and parasite specificity. We employed the bacterium Pseudomonas fluorescens SBW25 and its DNA phage Phi2 to investigate the role of coevolution in the evolution of phage infectivity range and its relation with phage growth rate. At the phage population level, coevolution led to the evolution of broader infectivity range, but without an associated decrease in phage growth rate relative to the ancestor, whereas phage evolution in the absence of bacterial evolution led to an increased growth rate but no increase in infectivity range. In contrast, both selection regimes led to phage adaptation (in terms of growth rates) to their respective bacterial hosts. At the level of individual phage genotypes, coevolution resulted in within-population diversification in generalist and specialist infectivity range types. This pattern was consistent with a multilocus gene-for-gene interaction, further confirmed by an observed cost of broad infectivity range for individual phage. Moreover, coevolution led to the emergence of bacterial genotype by phage genotype interactions in the reduction of bacterial growth rate by phage. Our study demonstrates that the strong reciprocal selective pressures underlying the process of coevolution lead to the emergence and coexistence of different strategies within populations and to specialization between selective environments. 相似文献
105.
C. Vacher D. Bourguet F. Rousset C. Chevillon M. E. Hochberg 《Journal of evolutionary biology》2004,17(4):913-918
Cultivating non‐toxic conventional crops (refuges) in the proximity to transgenic crops that produce Bacillus thuringienesis (Bt) toxins is widely recommended to delay pest adaptation to these toxins. Using a spatially structured model of resistance evolution, Vacher and co‐workers (Vacher, C., Bourguet, D., Rousset, F., Chevillon, C. & Hochberg, M.E. 2003. J. Evol. Biol. 16 : 378–387.) show that the percentage of refuge fields required for the sustainable control of pests can be reduced through intermediate levels of refuge field aggregation and by lowering the toxin dose produced by Bt plants. Tabashnik, B.E., Gould, F. & Carrière, Y. (2004 J. Evol. Biol doi: 10.1111/j1420–9101.2004.00695.x) call into question the results of Vacher et al. (2003) concerning the effect of toxin dose. They argue that these results arise from invalid assumptions about larval concentration–mortality responses for the insect considered, the cotton pest Heliothis virescens. We show here that the models presented by Vacher et al. (2003) and Tabashnik et al. (2004) both show inaccuracies in their definitions of genotypic fitness. The level of dominance estimated by Tabashnik et al. (2004) from larval mortality rates data is irrelevant to resistance evolution, and the fitness cost of resistance evolution, and the fitness cost of resistance is inaccurately integrated into their framework. Neverthless, the comments of Tabashnik et al. (2004) are very helpful in elucidating the definitions of genotypic fitness used in Vacher et al. (2003) and in pointing out the essential factors in predicting the evolution of insect resistance to Bt transgenic crops, namely, accurate estimations of the fitness cost of resistance, of the dominance level of this cost, and of the variations in the dominance level of the advantage conferred by the resistance with Bt toxin dose. 相似文献
106.
Ilan Gielchinsky Michal Gilon Rasha Abu-lail Imad Matouk Avraham Hochberg Ofer N. Gofrit 《Biomarkers》2017,22(7):661-666
Context: Urothelial carcinoma (UC) is common and highly recurrent. Diagnosis and follow-up involve invasive cystoscopies.
Objective: To evaluate H19 RNA in urine cells as diagnostic tool for UC.
Materials and methods: RT-PCR analysis of urine samples from healthy volunteers and UC patients.
Results: H19 RNA was unequivocally detected in the urine of 90.5% of patients and 25.9% of controls. H19 copies were three orders of magnitude higher in patients. Receiver operating characteristic analysis showed an area under the curve of 0.933.
Conclusions: This pilot study shows that urinary cell H19 is a highly sensitive test for UC and pending verification could transform patient management. 相似文献
107.
Hochberg Uri Bonel Andrea Giulia David-Schwartz Rakefet Degu Asfaw Fait Aaron Cochard Hervé Peterlunger Enrico Herrera Jose Carlos 《Planta》2017,245(6):1091-1104
Planta - Drought-acclimated vines maintained higher gas exchange compared to irrigated controls under water deficit; this effect is associated with modified leaf turgor but not with improved... 相似文献
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We develop a simple mathematical model to investigate the question as to whether a specialised consumer can be responsible for creating a range limit in the population of its dynamic resource. The model is most attuned for parasitoid-host relationships, but the central results should apply to a broad range of systems. Specifically, at the beginning of each simulation host and parasitoid populations are distributed at random along a string of patches. In each discrete generation and for each patch, host and parasitoid populations grow and interact, and then a constant fraction of those remaining disperses one or more patch distances in either direction according to a geometric distribution. We iterate the model 200 generations, and in any generation for any patch, either host and/or parasitoid can go locally extinct if its population falls below a threshold density. We find that a specialised parasitoid can enforce a limit, and it is even more likely to fragment its host population. The two most important conditions for parasitoid-enforced range limits are: 1) the theoretical host equilibrium density in the presence of the parasitoid be very small at sites eliminated from the host's range, and 2) the parasitoid disperses at high rates. We close by discussing our findings for specialist and generalist natural enemies, and the relevance of our study to the wealth of investigations on the causes of geographical range limits. 相似文献